Public Release: 25-Aug-2015
German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ)
Noroviruses are the predominant cause of gastroenteritis outbreaks in community settings such as hospitals, cruise ships, and schools. The virus is extremely contagious and is mostly transmitted via “fecal-oral-route”, i.e., through contaminated hands or contaminated food. Symptoms include violent and sudden onset of diarrhea, vomiting, and nausea.
“It is therefore important to provide a safe and harmless disinfectant against human norovirus,” explains Grant Hansman, head of CHS junior research group at the German Cancer Research Center noroviruses and the University of Heidelberg. The group is funded by the CHS Foundation. Some older observations found that fruit extracts, such as orange or pomegranate juice, could reduce the infectivity of surrogate noroviruses.
This current study was a continuation of an earlier project conducted at the National Institutes of Health in the United States, where they discovered that citrate from a commercial company could bind to the norovirus capsid protein. “However, it was only by chance that this discovery was made, like a lot of scientific research, but it turns out to be very interesting.” Dr Hansman explains.
Human noroviruses does not grow in cell culture, so they tested the effects of increasing concentrations of citric buffer on the so called norovirus virus-like particles, which have the same surface characteristics as real viruses. Dr Hansman and his staff show that the virus particles change their shape after citrate binding. X-ray crystal structures revealed that the citrate – from lemon juice or citrate disinfectants – precisely interacts at the binding pocket on the particle that is involved in attaching to host ligands – the so-called histo-blood group antigens.
These new results may explain why citrate reduces the infectivity of noroviruses. “Maybe a few drops of lemon juice on contaminated food or surfaces may prevent the transmission of these viruses,” speculates Hansman. With his staff, he now plans to investigate if citric acid could reduce symptoms in those already infected with noroviruses.
Anna D. Koromyslova, Peter White, and Grant S. Hansman: Citrate alters norovirus particle morphology. Virology 2015, DOI: 10.1016/j.virol.2015.07.009
The German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) with its more than 3,000 employees is the largest biomedical research institute in Germany. At DKFZ, more than 1,000 scientists investigate how cancer develops, identify cancer risk factors and endeavor to find new strategies to prevent people from getting cancer. They develop novel approaches to make tumor diagnosis more precise and treatment of cancer patients more successful. The staff of the Cancer Information Service (KID) offers information about the widespread disease of cancer for patients, their families, and the general public. Jointly with Heidelberg University Hospital, DKFZ has established the National Center for Tumor Diseases (NCT) Heidelberg, where promising approaches from cancer research are translated into the clinic. In the German Consortium for Translational Cancer Research (DKTK), one of six German Centers for Health Research, DKFZ maintains translational centers at seven university partnering sites. Combining excellent university hospitals with high-profile research at a Helmholtz Center is an important contribution to improving the chances of cancer patients. DKFZ is a member of the Helmholtz Association of National Research Centers, with ninety percent of its funding coming from the German Federal Ministry of Education and Research and the remaining ten percent from the State of Baden-Württemberg.
Categories: . Defensive Medicine